Directed Evolution
Produktnummer:
1814b094c738e648cc8345ffcf1ad691c3
| Themengebiete: | De novo protein design SelFi computationally guided protein stapling gene synthesis mutagenesis |
|---|---|
| Veröffentlichungsdatum: | 23.06.2023 |
| EAN: | 9781071621547 |
| Sprache: | Englisch |
| Seitenzahl: | 278 |
| Produktart: | Kartoniert / Broschiert |
| Herausgeber: | Currin, Andrew Swainston, Neil |
| Verlag: | Springer US |
| Untertitel: | Methods and Protocols |
Produktinformationen "Directed Evolution"
This volume explores the latest techniques used by researchers to study directed evolution (DE) at each stage of the Design-Build-Test-Learn cycle. Chapters in this book cover topics such as designing overlap extension PCR primers for protein mutagenesis; antha-guided automation of Darwin assembly for the construction of bespoke gene libraries; rapid cloning of random mutagenesis libraries using PTO-Quickstep; and DE of glycosyltransferases by a single-cell screening method. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Directed Evolution: Methods and Protocols is a valuable resource for scientists and researchers who are interested in learning more about this field and incorporating these studies into new experimental workflows.
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