Cellular Quiescence
Produktnummer:
18e1c54de5c3774c7bae3e4648072f87f4
Themengebiete: | Cellular dormancy Eukaryotes Genomic regulation Prokaryotes Stem cells |
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Veröffentlichungsdatum: | 11.09.2018 |
EAN: | 9781493984657 |
Sprache: | Englisch |
Seitenzahl: | 303 |
Produktart: | Kartoniert / Broschiert |
Herausgeber: | Lacorazza, H. Daniel |
Verlag: | Springer US |
Untertitel: | Methods and Protocols |
Produktinformationen "Cellular Quiescence"
This detailed volume explores methods and protocols that aim to increase our understanding of how cells enter a quiescent state during homeostasis and how cells exit quiescence and re-enter differentiating cell divisions to restore damaged tissues, essential for developing new approaches in regenerative medicine in the future. The chapters in this book were designed to address cellular quiescence in prokaryote and eukaryote organisms, detection of quiescence (Hoechst/pyronin Y, FUCCI, CFSE, BrdU, H2B-GFP, CyTOF), quiescence in stem cells (skin, intestinal, neuronal, hematopoietic), genomic regulation (gene expression, transcription factors, lncRNA, RNA methylation), as well as analysis of the heterogeneity of quiescence by computer modeling. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, andtips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Cellular Quiescence: Methods and Protocols offers a broad view of basic and cutting-edge technology to inspire research in this emerging field of cell biology.

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